This reagent is for research use only: This kit is used to determine the content of serotonin (5-HT) in rat serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of rat serotonin (5-HT) in the specimen. The microplate was coated with purified rat serotonin (5-HT) antibody to prepare a solid phase antibody, and serotonin (5-HT) and then HRP-labeled 5 were sequentially added to the microcapsules of the coated monoclonal antibody. The serotonin (5-HT) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with serotonin (5-HT) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat serotonin (5-HT) in the sample was calculated from a standard curve.

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